WebThermo Scientific MluI restriction enzyme recognizes A^CGCGT sites and cuts best at 37°C in R buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme … Thermo Scientific FastDigest MluI is one of an advanced line of fast restriction … Thermo Scientific MluI restriction enzyme recognizes A^CGCGT sites and cuts … Browse our complete online catalog of analytical instruments, laboratory … Web20 micrograms of the first-step cloning product was digested with MluI-HF and SphI-HF (New England Biolabs, Ipswich, MA), treated with Shrimp Alkaline Phosphatase, and purified with the QIAquick PCR purification kit. 2 micrograms of the purified product was ligated with 0.7 micrograms of PCR-amplified insert (described above) to give the final ...
Thermo Scientific™ MluI (10 U/µL) 10U/μL, 1000U Thermo Scientific™ MluI ...
Web30 jul. 2024 · To Request Technical Support Fill out our Technical Support Form , email us, or call 1-800-632-7799. For Questions Related to NEB Products and Offers Contact your local US Sales Representative . For Help With Your Order Contact our Customer Service Team by email or call 1-800-NEB-LABS. For Customers Outside of the US WebUse Thermo Scientific GeneJET PCR Purification Kit, #K0701 to purify PCR product prior digestion in following cases: When PCR additives such as DMSO or glycerol where … flashpoint oklahoma city tv show
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Web25 mrt. 2024 · Proper insertion and presence of the SpeI recognition site were confirmed by double digestion using HindIII (Thermo Scientific, no. ER0501)/MluI (Thermo Scientific, no. ER0562) and SpeI (Thermo ... WebCheck out videos that might be relevant for performing Restriction Enzymes MluI using FastDigest MluI from Thermo Fisher Scientific. Please note that these videos are … WebTo ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of … flashpoint omaha